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ccnd1 promoter luciferase reporter construct  (Addgene inc)


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    Structured Review

    Addgene inc ccnd1 promoter luciferase reporter construct
    Ccnd1 Promoter Luciferase Reporter Construct, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cyclin+d1+promoter/1748+human+cyclin+D1+promoter+pGL3Basic+(Plasmid+%2332726)/pm39729169-53-6-10
    Average 92 stars, based on 22 article reviews
    ccnd1 promoter luciferase reporter construct - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Activity Assay:

    Article Title: PARP14 regulates cyclin D1 expression to promote cell-cycle progression.
    Article Snippet: Cyclin D1 is an essential regulator of the G1–S cell-cycle transition and is overexpressed in many cancers.. Expression of cyclin D1 is under tight cellular regulation that is controlled by many signaling pathways.. Here we report that PARP14, a member of the poly (ADP-ribose) polymerase (PARP) family, is a regulator of cyclin D1 expression.

    Luciferase:

    Article Title: PARP14 regulates cyclin D1 expression to promote cell-cycle progression.
    Article Snippet: Cyclin D1 is an essential regulator of the G1–S cell-cycle transition and is overexpressed in many cancers.. Expression of cyclin D1 is under tight cellular regulation that is controlled by many signaling pathways.. Here we report that PARP14, a member of the poly (ADP-ribose) polymerase (PARP) family, is a regulator of cyclin D1 expression.

    Article Title: Activation of c-Myc and Cyclin D1 by JCV T-Antigen and β-Catenin in Colon Cancer
    Article Snippet: Cells were transfected using Xtreme Gene-HP (Roche). .. The following constructs were used for luciferase assays: M72 Super 16x TOPflash reporter construct: Addgene plasmid 17165; M51 Super 8x FOPflash: Addgene plasmid 12457; c-Myc promoter: Addgene plasmid 16595; Cyclin D1 promoter: Addgene plasmid 32727. .. These reporter constructs were transfected alone or together with pcDNA-T-Antigen and/or pcDNA-β-catenin and assayed for luciferase activity using a Luciferase Assay System (Promega) at 24 hours post-transfection.

    Plasmid Preparation:

    Article Title: PARP14 regulates cyclin D1 expression to promote cell-cycle progression.
    Article Snippet: Cyclin D1 is an essential regulator of the G1–S cell-cycle transition and is overexpressed in many cancers.. Expression of cyclin D1 is under tight cellular regulation that is controlled by many signaling pathways.. Here we report that PARP14, a member of the poly (ADP-ribose) polymerase (PARP) family, is a regulator of cyclin D1 expression.

    Article Title: Activation of c-Myc and Cyclin D1 by JCV T-Antigen and β-Catenin in Colon Cancer
    Article Snippet: Cells were transfected using Xtreme Gene-HP (Roche). .. The following constructs were used for luciferase assays: M72 Super 16x TOPflash reporter construct: Addgene plasmid 17165; M51 Super 8x FOPflash: Addgene plasmid 12457; c-Myc promoter: Addgene plasmid 16595; Cyclin D1 promoter: Addgene plasmid 32727. .. These reporter constructs were transfected alone or together with pcDNA-T-Antigen and/or pcDNA-β-catenin and assayed for luciferase activity using a Luciferase Assay System (Promega) at 24 hours post-transfection.

    Article Title: Transmembrane Protease Serine 4 Promotes Thyroid Cancer Proliferation via CREB Phosphorylation
    Article Snippet: .. The reporter plasmids containing wild-type (Plasmid #32727) and mutated CREB binding sites in the cyclin D1 promoter (Plasmid #32732) were purchased from Addgene (Cambridge, MA). .. The CREB reporter plasmid was created by inserting annealed oligonucleotides ( 18 ) 5′-C TGACGTCA GAG TGACGTCA GAG TGACGTCA GAG TGACGTCA A-3′ and 5′-GATCTTGACGTCACTCTGACGTCACTCTGACGTCACTCTGACGTCAGGTAC-3′ (the response element in the forward strand is underlined) into the Kpn I and Bgl II sites of the pTA-Luc vector (Clontech, Palo Alto, CA).

    Clone Assay:

    Article Title: PARP14 regulates cyclin D1 expression to promote cell-cycle progression.
    Article Snippet: Cyclin D1 is an essential regulator of the G1–S cell-cycle transition and is overexpressed in many cancers.. Expression of cyclin D1 is under tight cellular regulation that is controlled by many signaling pathways.. Here we report that PARP14, a member of the poly (ADP-ribose) polymerase (PARP) family, is a regulator of cyclin D1 expression.

    Construct:

    Article Title: Activation of c-Myc and Cyclin D1 by JCV T-Antigen and β-Catenin in Colon Cancer
    Article Snippet: Cells were transfected using Xtreme Gene-HP (Roche). .. The following constructs were used for luciferase assays: M72 Super 16x TOPflash reporter construct: Addgene plasmid 17165; M51 Super 8x FOPflash: Addgene plasmid 12457; c-Myc promoter: Addgene plasmid 16595; Cyclin D1 promoter: Addgene plasmid 32727. .. These reporter constructs were transfected alone or together with pcDNA-T-Antigen and/or pcDNA-β-catenin and assayed for luciferase activity using a Luciferase Assay System (Promega) at 24 hours post-transfection.

    Binding Assay:

    Article Title: Transmembrane Protease Serine 4 Promotes Thyroid Cancer Proliferation via CREB Phosphorylation
    Article Snippet: .. The reporter plasmids containing wild-type (Plasmid #32727) and mutated CREB binding sites in the cyclin D1 promoter (Plasmid #32732) were purchased from Addgene (Cambridge, MA). .. The CREB reporter plasmid was created by inserting annealed oligonucleotides ( 18 ) 5′-C TGACGTCA GAG TGACGTCA GAG TGACGTCA GAG TGACGTCA A-3′ and 5′-GATCTTGACGTCACTCTGACGTCACTCTGACGTCACTCTGACGTCAGGTAC-3′ (the response element in the forward strand is underlined) into the Kpn I and Bgl II sites of the pTA-Luc vector (Clontech, Palo Alto, CA).



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